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Related Experiment Video

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An investigation of downstream processing methods for challenging skeletal samples.

Jennifer L Snedeker1, Michelle A Peck2, David A Russell2

  • 1Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, TX, USA.

Forensic Science International. Genetics
|December 25, 2024
PubMed
Summary

Advanced genotyping methods successfully analyze challenging skeletal remains for unidentified human remains (UHRs). Next-generation sequencing and microarray analysis improve DNA profile recovery and enable forensic investigative genetic genealogy (FIGG).

Keywords:
Forensic investigative genetic genealogyGenotyping methodsHuman identificationNext generation sequencingSkeletal remainsUnidentified human remains

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Area of Science:

  • Forensic Science
  • Genetics
  • Anthropology

Background:

  • Skeletal remains are crucial for unidentified human remains (UHRs) but present challenges due to low DNA quantity, degradation, and inhibitors.
  • Traditional methods often yield limited genetic information from compromised skeletal samples, hindering investigations.

Purpose of the Study:

  • To evaluate the effectiveness of three advanced genotyping methods for analyzing compromised skeletal samples.
  • To compare genotype recovery and assess the investigative potential of targeted NGS (STRs/SNPs), targeted NGS (SNPs), and microarray SNP genotyping.

Main Methods:

  • Targeted next-generation sequencing (NGS) using ForenSeq® DNA Signature Prep chemistry (STRs and SNPs).
  • Targeted NGS using ForenSeq® Kintelligence kit (SNPs).
  • SNP genotyping via Infinium Global Screening Array (microarray).

Main Results:

  • All three advanced methods demonstrated success with challenging skeletal samples.
  • ForenSeq® DNA Signature Prep improved CODIS core loci recovery compared to traditional STR typing.
  • ForenSeq® Kintelligence and Infinium Global Screening Array yielded eligible profiles for forensic investigative genetic genealogy (FIGG).

Conclusions:

  • Advanced genotyping methods significantly enhance the ability to retrieve probative genetic information from skeletal remains.
  • A proposed workflow integrates NGS and microarray techniques for optimal processing of challenging UHR skeletal samples.
  • These methods offer promising avenues for advancing investigations involving unidentified human remains.