Enhancing colonic health with encapsulated grape seed anthocyanins: Oral capsule for Colon-targeted delivery

Grape seed anthocyanins (GSA) offer health benefits and protect against diseases, including colitis. Its unpleasant smell and instability prevent widespread application. Antisolvent pretreatment GSA was encapsulated in chitosan-phytic acid 3D gel network. SEM and X-ray diffraction results demonstrate that pretreatment reduces GSA particle size and exhibits amorphous structure. FTIR confirmed they were physically encapsulated and not covalently bound. Its subsequent simulations digestion and fermentation showed only 26.69 % upper digestive tract leakage and altered gut microbiota and metabolites profile. In DSS-induced colitis model, it ameliorated the symptoms, including diarrhea, bloody stools, weight loss, and DAI score. Additionally, it regulates colitis mice pro- and anti-inflammatory cytokines, modifies cecum and colon SCFA profile, improves intestinal barrier, and restores colonic cell redox equilibrium. Collectively, GSA ameliorates experimental colitis via inhibiting TRL4/NF-κB and activating Nrf2 signaling pathway. In conclusion, we propose our GSA capsule can effectively deliver an intact parent form of GSA to the colon and has the potential to be a colonic health strategy.