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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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In biostatistics, data are the observations collected for analysis. There are two main types: parametric and non-parametric. Parametric data, which include continuous (e.g., weight) and discrete numerical data (e.g., number of tablets), assume a particular distribution pattern, often the normal distribution. Non-parametric data do not adhere to a specific distribution and typically comprise nominal (e.g., gender) and ordinal categorical data (e.g., pain scale ratings).
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Data is the new gold

C Niek van Dijk1

  • 1Professor (Em) University of Amsterdam, Department of Orthopedic Surgery, Amsterdam UMC location AMC, the Netherlands; Ankle Unit, FIFA Medical Centre of Excellence Ripoll-DePrado Sport Clinic Madrid, Spain; Ankle Unit, FIFA Medical Centre of Excellence Clínica do Dragão Porto, Portugal; Casa di Cura San Rossore, Pisa, Italy.

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