Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

728
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
728
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

714
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
714
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

891
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
891
Mass Spectrometers01:16

Mass Spectrometers

5.2K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.2K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

630
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
630

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biocompatible and long-acting polyphosphate inhibitors for the diagnosis and treatment of thrombosis and thromboembolism.

Biomaterials advances·2026
Same author

Non-Destructive Extraction and Characterization of Human Pulmonary Microplastics and the Associated Endocrine Disrupting Chemicals.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

SMART (Shuimu Automated Reconstruction Technology): An Integrated Computational Platform for Streamlining Cryo-Electron Microscopy Workflow.

Journal of visualized experiments : JoVE·2026
Same author

Efficacy of robot-assisted stereotactic aspiration in moderate basal ganglia hemorrhage: a retrospective cohort study.

Frontiers in surgery·2026
Same author

Sex differences in aetiology of intracerebral haemorrhage and associated small vessel disease patterns.

European stroke journal·2026
Same author

Chemical Inertness Dominated Intrinsic Safety: Unraveling the "Dissolution-Catalysis-Runaway" Mechanism in Sodium-Ion Battery Cathode Materials.

The journal of physical chemistry letters·2026

Related Experiment Video

Updated: Jun 4, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K

Improved Point-of-Care Mass Spectrometry Analysis with Thin-Layer Chromatography-Based Two-Dimensional Separation and

Zisheng Ju1,2, Xiangyu Guo1, Linsen Li1,3

  • 1Chinese Academy of Inspection and Quarantine, Beijing 100176, China.

Analytical Chemistry
|December 26, 2024
PubMed
Summary

A new method combines thin-layer chromatography and miniature mass spectrometry for rapid point-of-care testing. This approach enables fast analysis of brain glioma biomarkers in clinical samples outside the lab.

More Related Videos

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.6K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K

Related Experiment Videos

Last Updated: Jun 4, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.6K
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

9.4K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Clinical Diagnostics

Background:

  • Point-of-care testing (POCT) provides rapid, on-site biochemical analysis, improving clinical decision-making.
  • Traditional laboratory testing involves sample transport and preparation delays.
  • Biomarkers like GABA, 2-HG, and NAA are crucial for diagnosing conditions such as brain gliomas.

Purpose of the Study:

  • To develop a novel methodology for rapid on-site clinical analysis.
  • To integrate thin-layer chromatography-based two-dimensional separation with miniature mass spectrometry.
  • To validate the method using known brain glioma biomarkers.

Main Methods:

  • Development of a novel analytical technique combining thin-layer chromatography (TLC) with miniature mass spectrometry (MS).
  • Utilized on-TLC derivatization and reactive spray ionization for enhanced MS signal detection.
  • Method validation using γ-aminobutyric acid (GABA), 2-hydroxyglutarate (2-HG), and N-acetyl-l-aspartic acid (NAA) as model analytes.

Main Results:

  • Achieved satisfactory analytical performance with a 1 ng/mL limit of detection and 2 ng/mL limit of quantitation.
  • Demonstrated high recoveries for the model analytes, ranging from 85.9% to 107.2%.
  • Successfully applied the method to analyze clinical samples, proving its potential for real-world applications.

Conclusions:

  • The integrated TLC-miniature MS approach offers a viable solution for rapid, on-site biochemical analysis.
  • The method shows significant promise for the early detection and monitoring of diseases like brain gliomas.
  • This technology has the potential to extend advanced analytical capabilities beyond traditional laboratory settings.