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Updated: Jun 4, 2025
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
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[Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization].
Jing-Bo Wang1, Xiao-Lan Li1, Rui-Xia Fan1
1West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Se Pu = Chinese Journal of Chromatography
|December 26, 2024
Summary
Nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI MSI) advances ion source development and lipid/protein analysis. This technique offers high sensitivity and spatial resolution for various samples, driving rapid growth in the field.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry Imaging
- Biotechnology
Background:
- Ambient mass spectrometry imaging (MSI) allows direct mapping of analytes in tissues at atmospheric pressure with minimal sample prep.
- Nanospray desorption electrospray ionization (nano-DESI) is a liquid-extraction-based ambient ionization technique, first used for MSI in 2012.
- nano-DESI MSI offers minimal sample preparation, high spatial resolution, and high sensitivity, suitable for diverse sample types.
Purpose of the Study:
- To review key nano-DESI MSI research progress over the past three years.
- To facilitate understanding and application of nano-DESI MSI technology.
- To highlight advances in ion-source development, lipid analysis, and spatial proteomics using nano-DESI MSI.
Main Methods:
- Review of recent advancements in nano-DESI ion source development and its coupling with various mass spectrometers.
- Analysis of novel nano-DESI MSI techniques for detailed lipid structure identification, including distinguishing isomers.
- Exploration of nano-DESI MSI applications in imaging intact protein assemblies and proteoforms.
Main Results:
- Significant progress in nano-DESI ion source design and successful coupling with diverse mass spectrometers (e.g., Orbitrap, Q Exactive, timsTOF Pro2).
- Enhanced capabilities in lipid analysis, enabling identification and imaging of complex lipid structures and isomers.
- Demonstrated success in spatial proteomics, including studying protein-ligand interactions and imaging endogenous proteoforms.
Conclusions:
- nano-DESI MSI is a rapidly developing technique with significant recent advancements in instrumentation and applications.
- The technique shows great promise for detailed molecular analysis in complex biological and environmental samples.
- Future research should focus on addressing challenges in ion-source development and enhancing instrumental performance for large biomolecules.
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