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Updated: May 8, 2025

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Light-Controlled Secretion and Injection of Proteins into Eukaryotic Cells by Optogenetic Control of the Bacterial
Florian Lindner1, Sebastian Grossmann1, Andreas Diepold2,3
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Abstract:
Gram-negative bacteria can use the type III secretion system (T3SS) to inject effector proteins into eukaryotic target cells. In this chapter, we describe the application of a light-controlled T3SS, based on the targeted sequestration of an essential dynamic T3SS component with the help of optogenetic interaction switches. This method enables to control the secretion or injection into eukaryotic cells for a wide range of protein cargos with high temporal and spatial precision.
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