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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

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DETECTION OF MECA AND NUC GENES OF MULTI-DRUG RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT CLINICAL

S Abood1, L Shihab1, G Abed2

  • 11College of Education, Al-Iraqia University, Baghdad, Iraq.

Georgian Medical News
|December 26, 2024
PubMed
Abstract

Insights

Multiple antibiotic-resistant Staphylococcus aureus strains were identified in clinical samples. All identified strains carried the mecA gene, contributing to their antibiotic resistance.

Area of Science:

  • Clinical Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Six isolates of multidrug-resistant Staphylococcus aureus were collected from clinical specimens in Baghdad between January and March 2023.
  • Isolates originated from burn swabs (3), urinary tract infections (2), and wound swabs (1).

Purpose of the Study:

  • To investigate the genetic basis of antibiotic resistance in clinical isolates of Staphylococcus aureus.
  • To detect the presence of specific resistance genes, mecA and nuc, in these isolates.

Main Methods:

  • Isolate identification and confirmation using the Vitek2 device.
  • Antibiotic susceptibility testing against ten antibiotics.
  • Conventional Polymerase Chain Reaction (PCR) for the detection of mecA and nuc genes.

Main Results:

  • All multidrug-resistant Staphylococcus aureus isolates (6/6) tested positive for the mecA gene.
  • The nuc gene was detected in only two isolates (Isolates No. 1 and No. 4).

Conclusions:

  • The presence of the mecA gene is strongly associated with antibiotic resistance in Staphylococcus aureus.
  • These findings highlight the role of specific resistance genes in the prevalence of multidrug-resistant bacteria in clinical settings.

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