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Related Experiment Videos

A two-dimensional scanning ocular fluorophotometer.

J W McLaren, R F Brubaker

    Investigative Ophthalmology & Visual Science
    |February 1, 1985
    PubMed
    Summary
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    Researchers developed a new two-dimensional scanning ocular fluorophotometer to measure fluorescein concentration in the eye. This device enables rapid, detailed analysis of fluorescein dynamics in the cornea and anterior chamber.

    Area of Science:

    • Ophthalmology
    • Biomedical Engineering
    • Optical Instrumentation

    Background:

    • Accurate measurement of fluorescein dynamics in the anterior eye is crucial for understanding ocular physiology and disease.
    • Existing methods may lack the spatial resolution or speed required for detailed regional analysis.

    Purpose of the Study:

    • To design and construct a novel two-dimensional scanning ocular fluorophotometer.
    • To enable simultaneous or rapid sequential measurement of fluorescein intensity in multiple ocular regions.
    • To assess fluorescein concentration in a horizontal section through the cornea and anterior chamber.

    Main Methods:

    • Utilized an argon laser (488 nm) for scanning the target area.
    • Employed a photon-counting photomultiplier tube for fluorescent light measurement.

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  • Developed a system capable of 30 anterior-posterior scans within 3 seconds, reconstructing a 2D fluorescein concentration array.
  • Achieved measurement sensitivity from 3 x 10(-10) gm/ml to 3 x 10(-6) g/ml.

    • Main Results:

    • Successfully constructed and tested a two-dimensional scanning ocular fluorophotometer.
    • Demonstrated the ability to measure fluorescein concentration with high sensitivity and spatial resolution.
    • Presented examples of scans revealing the "pupillary aqueous bubble" and radial corneal concentration gradients.

    Conclusions:

    • The developed instrument effectively measures regional fluorescein concentration differences in the anterior eye.
    • This technology facilitates detailed studies of fluorescein pharmacokinetics and ocular fluid dynamics.
    • The device offers valuable insights into drug delivery and physiological processes within the cornea and anterior chamber.