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Related Experiment Videos

Perfusion outflow facility in the rabbit eye. Stabilization by EACA.

J B Ruben, R A Moses, W J Grodzki

    Investigative Ophthalmology & Visual Science
    |February 1, 1985
    PubMed
    Summary
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    The serine protease inhibitor epsilon-aminocaproic acid (EACA) significantly reduces the washout effect in eye perfusion studies. This finding suggests EACA can improve aqueous humor dynamics research by minimizing perfusion-induced changes.

    Area of Science:

    • Ophthalmology
    • Physiology
    • Pharmacology

    Background:

    • Prolonged eye perfusion studies are limited by the washout effect, an increase in apparent outflow facility.
    • Understanding aqueous humor dynamics requires overcoming these perfusion-induced artifacts.

    Purpose of the Study:

    • To investigate the efficacy of epsilon-aminocaproic acid (EACA) in attenuating the washout effect during eye perfusion.
    • To determine if EACA can improve the reliability of aqueous humor dynamics studies.

    Main Methods:

    • Postmortem rabbit eyes were perfused at constant pressure using saline.
    • Perfusates either contained EACA (3.8 X 10(-3) M) or leucine as a control.
    • Outflow facility over time was measured, and washout slopes were calculated via linear regression.

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    Main Results:

    • Eyes perfused with EACA exhibited significantly lower washout slopes compared to control eyes (P < 0.001).
    • The addition of EACA dramatically attenuated the time-dependent increase in apparent outflow facility.
    • This indicates a reduction in the washout effect when EACA is included in the perfusate.

    Conclusions:

    • A significant component of the washout effect in eye perfusion may be mediated by fibrinolytic activity or other EACA-sensitive pathways.
    • EACA shows promise as an additive to perfusates for blunting the washout effect in prolonged eye perfusion studies.
    • This could enhance the accuracy and utility of research into aqueous humor dynamics.