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Dental Pulp Regeneration in Dogs Using a Chemokine Receptor 3 Antagonist Without Transplantation of Dental Pulp Stem
Koichiro Iohara1, Akiho Nagumo2, Michiyo Tominaga1
1Section of Regenerative Dental Medicine, National Center for Geriatrics and Gerontology, Research Institute, Obu, Aichi, Japan.
Introduction:
Our previous study showed that transplantation of dental pulp stem cells in combination with a chemokine receptor 3 (CCR3) antagonist into the root canals of aged dogs promoted dental pulp regeneration. In this study, we attempted to regenerate dental pulp in young dogs using a CCR3 antagonist without dental pulp stem cell transplantation.
Methods:
The teeth of dogs were histologically evaluated 4 weeks after extraction of the pulp and administration of scaffold materials and CCR3 antagonist (KDH-136) into the root canal. The effects of KDH-136 on the inflammatory response of RAW264.7 cells were investigated in vitro. In addition, we also examined whether KDH-136 affected neurite outgrowth of PC12 cells.
Results:
Application of KDH-136 resulted in the formation of dental pulp-like tissue. Furthermore, nerve and blood vessel regeneration were observed. KDH-136 suppressed chemokine production from RAW264.7 cells and promoted nerve growth factor-stimulated neurite outgrowth of PC12 cells.
Conclusions:
CCR3 antagonists may be useful for developing novel methods of dental pulp regeneration that do not require transplantation of dental pulp stem cells.
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