Anaerobic plasmalogen production in recombinant Escherichia coli carrying plasmalogen synthase gene from Selenomonas
Naoki Yoshii1, Keita Higuchi1, Tomoko Onodera2
1Department of Agricultural Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Bioscience, Biotechnology, and Biochemistry
|December 26, 2024
Abstract:
Escherichia coli expressing SrPlsAR from Selenomonas ruminantium produces plasmalogen, comprising almost 60% of the total phospholipid content under anaerobic conditions. Both plasmenylethanolamine and plasmenylglycerol were detected, and the major acyl aldehyde derived from sn-1 vinyl ether was C16:1. Plasmalogen synthesis is affected by mutations in ATP-binding sites and Cys is expected to be involved in the formation of the [4Fe-4S] cluster.


