Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Helicases00:55

DNA Helicases

21.1K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.1K
The DNA Replication Fork01:02

The DNA Replication Fork

35.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.5K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

13.9K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
13.9K
The Replisome03:01

The Replisome

33.0K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.0K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
DNA Topoisomerases02:02

DNA Topoisomerases

30.9K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
30.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

De novo design of DNA origami with a generative diffusion model.

Nature communications·2026
Same author

Classifying Multistate DNA Origami: An Automated Approach with Minimal Labeling and Confidence-Based Filtering.

Journal of chemical information and modeling·2026
Same author

Uncovering Design and Assembly Rules for mRNA-DNA Origami.

Nano letters·2026
Same author

SNUPI: A Computational Framework for Rapid Mechanical Analysis of Structured DNA Assemblies.

JACS Au·2025
Same author

A long-staple design approach towards the scalable production of scaffolded DNA origami.

Nanoscale horizons·2025
Same author

A Computational Framework for Investigating the Mechanical Stresses on Breast Implants Under Dynamic Loading Conditions.

Annals of biomedical engineering·2025

Related Experiment Video

Updated: Jun 4, 2025

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.2K

A DNA Origami Pivot Hinge Driven by DNA Intercalators.

Taehwi Kim1, Seo Hyun Kwon1, Do-Nyun Kim1,2,3

  • 1Department of Mechanical Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea.

ACS Nano
|December 26, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a DNA origami hinge with continuous pivot motion controlled by DNA intercalators. This reversible nanoscale actuator can be used in developing advanced nanosensors and actuators.

Keywords:
DNA intercalatorDNA nanotechnologynanoactuatorpivot hinge mechanismreconfiguration

More Related Videos

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.5K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.0K

Related Experiment Videos

Last Updated: Jun 4, 2025

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.2K
Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.5K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.0K

Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • DNA origami enables the construction of reconfigurable nanoscale structures.
  • Dynamic control over nanoscale component movement is crucial for advanced applications.

Purpose of the Study:

  • To propose and demonstrate a novel DNA origami hinge with continuous, controllable pivot motion.
  • To explore the use of DNA intercalators for actuating nanoscale movements.

Main Methods:

  • Constructed a DNA origami hinge comprising two six-helix bundles linked by gold nanoparticles.
  • Utilized varying concentrations and types of DNA intercalators to control pivot motion.
  • Investigated the effect of gold nanoparticle distance on motion sensitivity and range.

Main Results:

  • Achieved continuous, reversible, and repeatable pivot motion in the DNA origami hinge.
  • Demonstrated tunability of motion sensitivity and range by adjusting intercalator type and nanoparticle spacing.
  • Successfully demonstrated a concept for bending nanoactuators by connecting three pivot hinges.

Conclusions:

  • The proposed DNA origami pivot hinge offers a novel mechanism for nanoscale actuation.
  • This technology holds potential for developing sensitive nanosensors and actuators that amplify minute molecular interactions.