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Related Experiment Video

Updated: Jun 4, 2025

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing
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Functional and Structural Improvement of Engineered Cardiac Microtissue Using Aligned Microfilaments Scaffold.

Mohammad Karami1,2, Hamid Keshvari1, Mohammad Amin Hajari2

  • 1Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 159163-4311, Iran.

ACS Biomaterials Science & Engineering
|December 26, 2024
PubMed
Summary

Engineered cardiac microtissue using aligned microfilaments improves cell structure and function. This novel scaffold enhances cellular alignment and synchronized electrophysiological signals for better cardiovascular disease modeling.

Keywords:
cardiac microtissuecellular alignmentcoculturemicrofilament scaffoldtissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Cardiovascular Research
  • Tissue Engineering

Background:

  • Reliable in vitro preclinical systems are crucial for advancing cardiovascular disease therapeutics.
  • Current models often lack the necessary 3D architecture to accurately mimic native heart tissue.
  • Improved disease modeling and drug testing require more physiologically relevant engineered heart tissues.

Purpose of the Study:

  • To engineer a novel scaffold for cardiac microtissues that promotes cellular alignment.
  • To investigate the impact of aligned microfilaments on cellular structure and tissue organization.
  • To assess the functional improvements in engineered cardiac microtissue.

Main Methods:

  • Fabrication of a scaffold using aligned poly(lactic-co-glycolic acid) (PLGA) microfilaments.
  • Coculture of cardiac progenitor cells (CPC), human umbilical cord endothelial cells (HUVEC), and human foreskin fibroblasts (HFF) within the 3D scaffold.
  • Assessment of cellular alignment, cytoskeletal organization (F-actin staining), and electrophysiological function.

Main Results:

  • The engineered cardiac microtissue (ECMT) with aligned microfilament scaffold (AMFS) demonstrated significantly improved cellular alignment compared to controls.
  • Cells within the ECMT exhibited enhanced uniaxial anisotropic and oriented cytoskeletons.
  • Synchronized electrophysiological signals indicated improved functional integration of the engineered tissue.

Conclusions:

  • The AMFS effectively induces cellular alignment and improves the structural and functional properties of ECMT.
  • This engineered cardiac microtissue represents a promising preclinical model for cardiovascular research.
  • The developed system holds potential for pharmaceutical research and broader biomedical applications.