PEDOT: PSS-Modified Organic Flexible and Implantable Microelectrode for Internal Bi-Directional Electrophysiology of
Qunchen Yuan1,2, Chunlian Qin3, Dongxin Xu3
1Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.
Abstract:
Three-dimensional (3D) cardiomyocyte spheroids are essential models to replicate cardiac structural and functional features in vitro. However, conventional planar and rigid microelectrode arrays (MEAs) suffer from low-quality electrophysiological recording of 3D cultures, due to limited contact areas and weak coupling between cells and MEA chips. Herein, we developed a PEDOT: PSS-modified organic flexible and implantable MEA (OFI-MEA) coupled with a self-developed integrated biosensing platform to achieve high-throughput, long-term, and stable bidirectional internal electrophysiology in 3D cardiomyocyte spheroids. Electrostimulation enhanced the functional performance of the 3D cardiomyocytes, causing a remarkable 2.69-fold increase in frequency. Furthermore, time-frequency analysis of the multisite electrophysiological signals to highlight diverse cell activity patterns in the spheroids. It provides a powerful tool to record electrophysiological signals of 3D cardiomyocyte spheroids, allowing continuing evaluation of cardiac dynamics and regulation of electrical signals, providing a novel evaluation strategy for cardiac disease model construction, drug screening, and cardiological research.
