Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated deep learning and multi-scale modeling for the discovery of pan-genotypic HCV NS5B polymerase inhibitors.

Molecular diversity·2026
Same author

Genetic regulation of stem elongation and thickening in ornamental plants: a review.

Horticulture research·2026
Same author

Determinants of Bird Richness in the Louzishan National Nature Reserve: Effects of Productivity and Habitat Heterogeneity Across Taxonomic and Functional Dimensions.

Ecology and evolution·2026
Same author

Multifocal Pixel/Photon-Reassignment FLIM (MPPR-FLIM): A Super-Resolution Analytical Tool for Characterizing Subcellular Fluorescence Lifetime Heterogeneity via TCSPC.

Analytical chemistry·2026
Same author

Deep-learning-assisted scattering structured-illumination confocal microscopy for industrial super-resolution imaging.

Optics express·2026
Same author

In Vivo Hyperspectral CARS Imaging Reveals Photobiomodulation-Driven Remodeling of Fatty Acid Homeostasis in an AD Mouse Model.

Analytical chemistry·2026

Related Experiment Video

Updated: Jun 4, 2025

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

7.7K

Multidimensional Characterization of the Physiological State of Hematococcuspluvialis Using Scanning Structured

Meiting Wang1, Yifeng Deng2, Yuye Wang2

  • 1School of Mechanical and Electrical Engineering, Guangdong University of Science and Technology, Dongguan 523083, China.

Analytical Chemistry
|December 27, 2024
PubMed
Summary

Scanning structured illumination microscopy revealed how Haematococcus pluvialis (HP) accumulates astaxanthin under stress. Light stress accelerates astaxanthin production more than salt stress by causing greater cellular disruption.

More Related Videos

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
09:45

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Published on: January 25, 2017

19.4K
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

17.8K

Related Experiment Videos

Last Updated: Jun 4, 2025

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
07:50

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

7.7K
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
09:45

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Published on: January 25, 2017

19.4K
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

17.8K

Area of Science:

  • Microscopy and Imaging
  • Cell Biology
  • Biochemistry

Background:

  • Haematococcus pluvialis (HP) is a microalga renowned for its high astaxanthin content, a valuable antioxidant.
  • Astaxanthin accumulation in HP is triggered by environmental stress, but the underlying mechanisms and cellular dynamics are not fully understood.
  • Existing imaging techniques are limited in their ability to provide super-resolution, label-free, and 3D visualization of HP stress responses.

Purpose of the Study:

  • To investigate the subcellular mechanisms of astaxanthin accumulation in HP under various stress conditions.
  • To utilize advanced imaging techniques for dynamic 3D ultrastructural reconstructions of HP cells.
  • To correlate morphological changes and cell wall alterations with the rate of astaxanthin accumulation.

Main Methods:

  • Employed scanning structured illumination microscopy (SSIM) for high-resolution, dynamic 3D imaging of HP cells.
  • Subjected HP cultures to different stress conditions (light and salt stress).
  • Analyzed cellular morphology, cell wall structure, and astaxanthin accumulation rates under identical stress durations.

Main Results:

  • SSIM enabled detailed observation of HP cell ultrastructure and stress-induced morphological changes.
  • Light stress caused more comprehensive cellular disruption compared to salt stress, which acted from the exterior inward.
  • The rate of astaxanthin accumulation under light stress was approximately double that observed under salt stress.

Conclusions:

  • Super-resolution microscopy (SSIM) is effective for elucidating the dynamics of astaxanthin accumulation in HP.
  • The extent of cellular disruption correlates with the rate of astaxanthin biosynthesis.
  • Findings provide novel insights into the subcellular processes governing astaxanthin production in response to environmental cues.