Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET...

Area of Science:

• Analytical Chemistry
• Nanotechnology
• Biochemistry

Background:

• Accurate thiamine monitoring is crucial for multivitamin quality and preventing deficiencies.
• Existing methods may lack selectivity or sensitivity for complex matrices like supplements.
• Novel sensing platforms are needed for reliable thiamine quantification.

Purpose of the Study:

• To develop a sensitive and selective Förster resonance energy transfer (FRET) system for thiamine detection.
• To optimize the synthesis of silver nanoparticles (AgNPs) for efficient FRET applications.
• To validate the method's accuracy and robustness in multivitamin supplements.

Main Methods:

• Aqueous synthesis of citrate-stabilized silver nanoparticles (AgNPs) using microwave irradiation.
• Optimization of AgNP synthesis using a central composite orthogonal design (CCOD).
• Development of a FRET sensing scheme utilizing carbon dots (CDs) and AgNPs for thiamine quantification, coupled with unfolded partial least-squares (U-PLS) and residual bilinearization (RBL) for data analysis.

Main Results:

• Optimized AgNP synthesis yielded efficient plasmonic nanoparticles with maximal absorbance, stability, and wavelength alignment.
• The FRET system demonstrated a concentration-dependent decrease in photoluminescence (PL) upon thiamine presence.
• U-PLS and RBL analysis achieved high sensitivity and specificity, with R² = 0.952 and REP% = 11% for thiamine detection.

Conclusions:

• A novel, highly sensitive, and interference-free FRET-based method for thiamine detection was successfully developed.
• The optimized AgNP synthesis and advanced data analysis provide a robust platform for analytical applications.
• This method holds significant potential for quality control of multivitamin supplements and other relevant analyses.