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Transcriptomic Profiling Reveals Altered Expression of Genes Involved in Metabolic and Immune Processes in
Malarmathi Muthusamy1, Kannaki T Ramasamy2, Sunday Olusola Peters3
1Department of Animal Genetics and Breeding, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Namakkal 637002, India.
Metabolites
|December 27, 2024
Summary
Newcastle Disease Virus (NDV) infection significantly alters gene expression in chicken embryos, impacting immune and metabolic pathways. Understanding these changes is key to improving poultry health and disease resistance.
Area of Science:
- Poultry Science
- Virology
- Genomics
Background:
- Viral infections, particularly Newcastle Disease Virus (NDV), pose significant economic threats to the poultry industry.
- Understanding developmental impacts and early infection effects on immune responses is crucial for poultry health.
Purpose of the Study:
- To investigate host-pathogen interactions in chicken embryos infected with NDV.
- To analyze gene expression changes in response to NDV infection.
Main Methods:
- Transcriptome analysis using RNA-Seq on NDV-infected chicken embryo lung tissues.
- Alignment of RNA-Seq reads to the chicken reference genome (Galgal7).
- Gene Ontology and KEGG pathway analyses to identify affected biological processes.
Main Results:
- 594 differentially expressed genes identified: 264 upregulated and 330 downregulated.
- Significant alterations in immune function, metabolism, cell cycle, and nucleic acid processes observed.
- Upregulation of immune-related genes (e.g., TLR4, TLR2, IL1R2) and metabolic genes (e.g., ALDOB, PRPS2), alongside novel transcripts and lncRNAs.
Conclusions:
- Gene expression changes are critical for regulating viral replication and immune responses during NDV infection.
- Identified genes offer potential targets for improving poultry breed resistance to NDV.
