Multifunctional SERS Chip for Biological Application Realized by Double Fano Resonance

Area of Science:

• Nanophotonics
• Spectroscopy
• Biotechnology

Background:

• Label-free, in situ molecular detection in biological cells is challenging due to weak Raman signals.
• Surface-Enhanced Raman Spectroscopy (SERS) offers signal enhancement but faces limitations with varying refractive indices and thermal sensitivity in cell culture media.

Purpose of the Study:

• To develop a SERS chip with integrated dual Fano resonances for enhanced, stable, and reliable detection of bioactive samples.
• To address the limitations of SERS in biological environments, including refractive index sensitivity and thermal damage.

Main Methods:

• Proposed a SERS chip design featuring integrated dual Fano resonances.
• Developed an analytical model for parameter optimization and performance analysis of the SERS chip.
• Conducted simulation and experimental characterization to validate the chip's performance.

Main Results:

• Demonstrated independent broadband tuning of dual Fano resonances.
• Showcased simultaneous enhancement of Raman signal excitation and radiation processes.
• Confirmed refractive index insensitivity and reduced heat generation.
• Observed synergistic enhancement of Raman signal amplitude and range.

Conclusions:

• The dual Fano resonance SERS chip provides a stable, reliable, and comprehensive tool for bioactive sample fingerprint detection.
• The developed chip overcomes key limitations of conventional SERS in biological applications.
• This technology advances in situ and label-free molecular analysis in cellular environments.