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Related Experiment Videos

Modulation of the platelet-derived growth factor induced replicative response.

C D Scher, A P Whipple, J P Singh

    Journal of Cellular Physiology
    |April 1, 1985
    PubMed
    Summary

    Platelet-derived growth factor (PDGF) sensitization in BALB/c-3T3 cells enhances mitogenesis. This occurs not by increased PDGF binding, but by cell loss, increasing PDGF per cell and promoting DNA synthesis.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Density-arrested BALB/c-3T3 cells are typically quiescent.
    • Platelet-derived growth factor (PDGF) is a key mitogen for these cells.

    Purpose of the Study:

    • To investigate the mechanism of PDGF sensitization in BALB/c-3T3 cells.
    • To understand how sensitization affects PDGF-mediated mitogenesis and protein synthesis.

    Main Methods:

    • Cell culture of BALB/c-3T3 cells.
    • Induction of quiescence and sensitization through serum/PDGF deprivation.
    • Measurement of PDGF binding, DNA synthesis, and major excreted protein (MEP) synthesis.

    Main Results:

    • Sensitized cultures required less PDGF for growth stimulation and MEP synthesis.

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  • Sensitization did not increase PDGF binding but resulted from cell loss during deprivation.
  • Increased PDGF per cell, due to cell loss, promoted higher rates of DNA synthesis.

    • Conclusions:

    • The amount of PDGF per cell, rather than its concentration, modulates mitogenesis.
    • Cell loss during PDGF deprivation is a key factor in sensitization, increasing the mitogenic effect of PDGF.