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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Updated: Jun 26, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

AptaFluorescence: An aptamer-based fluorescent imaging protocol for biomolecule visualization.

Crystal Chia Yin Ling¹, Melissa Jane Fullwood¹

¹School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

|December 27, 2024

Summary

This summary is machine-generated.

A novel aptamer-based method, AptaFluorescence, offers distinct visualization of biomolecules like c-MYC. This technique provides a valuable alternative to antibodies, especially for challenging targets in biological research.

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Last Updated: Jun 26, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

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Published on: September 15, 2020

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Immunofluorescence relies on antibody-antigen interactions for cellular biomolecule visualization.
Challenges exist in obtaining specific and high-affinity antibodies for certain protein targets.
Complex or low-abundance proteins are particularly difficult to target effectively with antibodies.

Purpose of the Study:

To develop a novel aptamer-based protocol for in vitro biomolecule visualization.
To address limitations associated with antibody-based detection methods.
To provide an alternative for visualizing biomolecules when specific antibodies are unavailable.

Main Methods:

Developed AptaFluorescence, a protocol using fluorescently labeled aptamers for biomolecule detection.
Aptamers are nucleic acid molecules with high specificity for target biomolecules.
Evaluated AptaFluorescence targeting the c-MYC protein in engineered U2OS cells.

Main Results:

AptaFluorescence provided more distinct signals compared to traditional immunofluorescence.
The protocol successfully differentiated between cells with and without c-MYC expression.
Demonstrated reliable visualization of the c-MYC protein.

Conclusions:

AptaFluorescence is a novel, user-friendly protocol for aptamer-based biomolecule visualization.
This method offers broad applicability in biological research.
AptaFluorescence is particularly useful when specific antibodies are lacking.