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Insulin binding to human ovaries.

J C Jarrett, G Ballejo, J C Tsibris

    The Journal of Clinical Endocrinology and Metabolism
    |March 1, 1985
    PubMed
    Summary
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    Researchers found high-affinity insulin binding sites in human ovarian plasma membranes. These binding characteristics remained consistent throughout the menstrual cycle, indicating a stable interaction.

    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Molecular Endocrinology

    Background:

    • Insulin plays a role in various physiological processes beyond glucose metabolism.
    • The presence and characteristics of insulin receptors in human ovarian tissue are not fully elucidated.

    Purpose of the Study:

    • To investigate the presence and binding characteristics of insulin receptors in human ovarian plasma membranes.
    • To determine if insulin receptor affinity or number varies during the menstrual cycle.

    Main Methods:

    • Human ovarian tissue samples were collected during laparotomy.
    • Plasma membrane fractions were isolated and used for receptor binding assays.
    • Radioligand binding with [125I]insulin (porcine) and Scatchard analysis were performed.

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    Main Results:

    • Scatchard analysis revealed biphasic binding curves, indicating multiple affinity sites.
    • High-affinity insulin binding sites were identified with an average concentration of 57.4 +/- 7.9 fmol/mg protein.
    • The dissociation constant for high-affinity sites was 3.5 +/- 0.9 nM, with no significant changes observed across the menstrual cycle.

    Conclusions:

    • Human ovarian plasma membranes possess high-affinity insulin binding sites.
    • The affinity and number of these insulin binding sites are stable throughout the menstrual cycle.
    • These findings suggest a potential direct role for insulin in human ovarian function.