Cytotoxicity and Bone Biocompatibility of the C-Root SP Experimental Root Canal Sealer

This study evaluated the cytotoxicity and biocompatibility of a new strontium silicate-based root canal sealer (C-Root SP), in comparison with those of iRoot SP and AH plus. The sealer extract was diluted to the concentrations of 100%, 75%, 50%, and 25%. L929 cells were cultured for 24 h, and the absorbance value was determined. Two-way analysis of variance (two-way ANOVA) and sealers were implanted in the tibia of Sprague-Dawley (SD) rats, At 2, 6, and 12 weeks, rats were euthanised. The tissue reaction was evaluated by HE staining. The least significant difference (LSD) t-test and Kruskal-Wallis test were used. The cytotoxicity of C-Root SP and iRoot SP was found to be less than that of AH plus. At 12 weeks, new bone formation was induced around C-Root SP and iRoot SP sealer, but minimal evidence of bone formation was found in AH plus. C-Root SP has low cytotoxicity and superior biocompatibility.