Mitochondrial transplantation reconstructs the oxidative microenvironment within fibroblasts to reverse photoaging

Fibroblast-mediated oxidative stress is a pivotal factor in the pathogenesis of skin photoaging, predominantly induced by UVA radiation. Diverging from traditional strategies that concentrate on the reduction of reactive oxygen species (ROS), the present study implements mitochondrial transplantation as an innovative therapeutic approach. The objective of this study is to reestablish the oxidative microenvironment and to effectively rejuvenate cellular functionality through the direct introduction of healthy and vibrant mitochondria. In vitro assays have illustrated that the seamless incorporation of exogenous mitochondria into fibroblasts ameliorates UVA radiation perturbations in membrane potential and oxidative stress, while simultaneously reestablishing the oxidative microenvironment. These interventions exert salutary influences on cellular proliferation and migratory capabilities. Subsequent in vivo analyses reveal a mitigation in dermal collagen depletion, alongside an enhancement in collagen fiber density and tissue architecture post-mitochondrial transplantation, thus ameliorating the manifestations of skin photoaging. Collectively, the study underscores the potential of mitochondrial transplantation as a promising therapeutic intervention for the reversal of skin photoaging by modulating the oxidative microenvironment within fibroblasts.