JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Metabolomic and transcriptomic analysis reveals metabolic-immune interactions in choroid neovascularization

  • 0Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China; Key Laboratory of Myopia and Related Eye Diseases, NHC, Chinese Academy of Medical Sciences, 83 Fenyang Road, Shanghai, 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, 83 Fenyang Road, Shanghai, 200031, China.
Experimental Eye Research +

|

December 28, 2024

|

December 28, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study reveals key immune and metabolic changes in choroidal neovascularization (CNV), a major cause of vision loss. Identifying interactions between immune cells and riboflavin metabolism offers new therapeutic targets for age-related macular degeneration (AMD).

Area Of Science

  • Ophthalmology
  • Immunology
  • Metabolomics
  • Transcriptomics

Background

  • Choroidal neovascularization (CNV) is a severe form of age-related macular degeneration (AMD) leading to significant vision loss in the elderly.
  • The laser-induced CNV model is crucial for studying the mechanisms of this condition.

Purpose Of The Study

  • To conduct an integrated metabolomic and transcriptomic analysis of CNV samples.
  • To identify key metabolic and immune signatures and their interplay during angiogenesis in CNV.
  • To explore potential therapeutic targets for neovascular AMD.

Main Methods

  • Integrated analysis of metabolomic and transcriptomic data from CNV samples.
  • Utilized single-sample gene set enrichment analysis (ssGSEA), correlation analysis, and weighted gene co-expression network analysis (WGCNA).
  • Employed various bioinformatics platforms for comprehensive data interpretation.

Main Results

  • Detected dominant infiltration of macrophages and monocytes in CNV samples.
  • Characterized a positive correlation between dysregulated riboflavin metabolism and angiogenesis pathways.
  • Identified Enpp1 and ACP5 as potential central regulators of immune-metabolic crosstalk in CNV.

Conclusions

  • The study classifies the immune and metabolic landscape in CNV models, highlighting critical interactions.
  • Enhanced understanding of neovascular AMD pathogenesis.
  • Provides a foundation for developing multi-targeted therapies for CNV and other neovascular eye diseases.

Keywords: