LPL-RH suppresses bone loss in ovariectomised rat models.

Area of Science:

• Microbiology
• Immunology
• Bone Biology

Background:

• Oestrogen deprivation leads to microbiota-dependent osteolysis.
• Probiotics show potential for treating this condition, but mechanisms need clarification.

Purpose of the Study:

• To provide evidence for probiotics as an adjuvant treatment for osteolysis.
• To explore the pathophysiology of oestrogen-deprived osteolysis.

Main Methods:

• Ovariectomised Sprague-Dawley rats were treated with probiotics (Bifidobacterium animalis subsp. lactis LPL-RH), calcium, or both.
• Osteometabolic effects and mechanistic roles of probiotics were evaluated.
• 16S rRNA sequencing analyzed changes in the gut microbiome.

Main Results:

• LPL-RH suppressed osteoclast activation and bone calcium loss by downregulating key signaling pathways (RANKL/TRAF6/NF-κB/NFATc1/TRAP).
• LPL-RH reduced pro-osteoclastogenic T cells (TH17), inflammatory molecules (LPS), and improved intestinal barrier function.
• LPL-RH altered the gut microbiome, reducing S24-7 and promoting Prevotella and Bacteroides.

Conclusions:

• Bifidobacterium animalis subsp. lactis LPL-RH suppresses osteoclastogenesis and osteolysis.
• LPL-RH achieves this by modulating type 17 immunity and the gut microbiome.