Comprehensive Mass Spectral Libraries of Human Thyroid Tissues and Cells

Area of Science:

• Endocrinology
• Proteomics
• Biomarker Discovery

Background:

• Thyroid nodules are common endocrine conditions with rising incidence.
• Proteomics using data-independent acquisition is key for identifying disease biomarkers.
• Analyzing the thyroid proteome requires high-throughput, reproducible methods.

Purpose of the Study:

• To create comprehensive thyroid-specific peptide spectral libraries.
• To enable high-throughput, reliable analysis of the thyroid disease proteome.
• To facilitate the discovery of thyroid disorder biomarkers and therapeutic targets.

Main Methods:

• Generated four deep-coverage spectral libraries using mass spectrometry (Q Exactive HF, Orbitrap Exploris 480, ZenoTOF 7600, timsTOF Pro).
• Libraries include over 215,000 precursors, 172,000 peptides, and 12,000 proteins.
• Data derived from 245 tissue samples (9 histological types) and 50 cell lines (6 histological types).

Main Results:

• Developed extensive peptide spectral libraries for thyroid research.
• Libraries cover a wide range of thyroid tissue and cell line proteomes.
• Successfully applied libraries to nine thyroid sample types for disease-specific protein exploration.

Conclusions:

• Thyroid-specific peptide spectral libraries are a valuable resource for proteomic analysis.
• These libraries enhance the exploration of disease-specific proteins in thyroid disorders.
• Facilitates deeper insights into the molecular mechanisms of thyroid diseases.