Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.1K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.1K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

3.7K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.7K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

5.8K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative analysis of nerve-sparing modified radical hysterectomy for deep endometriosis using the da Vinci SP versus Xi systems.

Journal of robotic surgery·2026
Same author

Comparison of surgical outcomes between robot-assisted and conventional laparoscopic hysterectomy for stage IV endometriosis: A propensity score-matched analysis and review of the literature.

Journal of robotic surgery·2026
Same author

Is Identifying the Ureter Using Indocyanine Green in Severe Deep Endometriosis Always Useful?

Journal of minimally invasive gynecology·2026
Same author

Boundary-shape driven transitions in vortex and oscillatory dynamics of confined epithelial cells.

Soft matter·2026
Same author

Force transmission in an actomyosin cytoskeletal network revealed by particle fluctuation analysis.

Biochemical and biophysical research communications·2025
Same author

Wetting-Mediated Remodeling of the Cytoskeletal Cortex by Phase-Separated Condensates in Cell-Free Reactions.

Biomacromolecules·2025
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature
08:04

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Published on: November 26, 2019

7.1K

Density-dependent flow generation in active cytoskeletal fluids.

Tomoka Kashiwabara1, Tatsuya Fukuyama1, Yusuke T Maeda2

  • 1Department of Chemical Engineering, Kyoto University, Nishi-kyoku, Kyoto, 615-8510, Japan.

Scientific Reports
|December 29, 2024
PubMed
Summary
This summary is machine-generated.

This study reveals how actomyosin cytoskeleton density influences cellular dynamics. We observed oscillating actin flows in cell extracts, driven by an interplay between actin density and myosin motor activity.

More Related Videos

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

727
Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
09:10

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Published on: August 25, 2022

3.0K

Related Experiment Videos

Last Updated: Jun 4, 2025

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature
08:04

Controlling Flow Speeds of Microtubule-Based 3D Active Fluids Using Temperature

Published on: November 26, 2019

7.1K
The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
08:50

The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Published on: March 10, 2023

727
Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
09:10

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Published on: August 25, 2022

3.0K

Area of Science:

  • Cellular dynamics
  • Biophysics
  • Cytoskeletal organization

Background:

  • The actomyosin cytoskeleton, composed of actin filaments and myosin motors, generates contractile forces crucial for cellular processes.
  • The precise relationship between actomyosin density and the resulting ordered structures and force generation remains unclear.

Purpose of the Study:

  • To investigate the density dependence of actomyosin cytoskeleton-driven flow generation.
  • To understand the emergent dynamics and force-controlled structures arising from actomyosin networks.

Main Methods:

  • Utilized cell-sized droplets containing purified actomyosin cytoskeleton and nucleation factors.
  • Varied the concentration of actomyosin to observe effects on actin flow and tracer particle motion.
  • Measured contraction-driven flow and analyzed oscillatory dynamics.

Main Results:

  • Observed continuous actin flow towards the center at a critical actomyosin density.
  • Discovered emergent oscillatory dynamics in actin flow, characterized by periodic stop-and-go motion of tracers.
  • Found that tracer particle velocity decreases with actomyosin density in the oscillatory regime, exhibiting superdiffusive behavior.
  • Demonstrated that alterations in myosin activity disrupt the regularity of oscillatory flow.

Conclusions:

  • Actomyosin density-dependent flow generation is governed by the interplay between actin concentration and myosin motor force.
  • Emergent oscillatory dynamics are a key feature of actomyosin networks at specific densities.
  • This work provides insights into the self-organization principles of cytoskeletal systems.