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A sound approach for ova denudation.

Amir Mokhtare1, Amirhossein Favakeh2, Philip Xie3

  • 1Department of Food Science, Cornell University, Stocking Hall, Ithaca, New York; Center for Technology Licensing, Cornell University, Ithaca, New York.

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|December 29, 2024
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Summary

A novel acoustohydrodynamic tweezers method offers a faster, safer, and reliable noncontact way to remove cumulus cells from oocytes for intracytoplasmic sperm injection (ICSI). This technique achieves comparable embryo development and live birth rates to conventional methods, reducing procedure time and hyaluronidase use.

Keywords:
Acoustic-assisted denudationICSIacoustohydrodynamic tweezerscumulus-oocyte-complexmicrofluidics

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Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Medical Devices

Background:

  • Conventional methods for denuding cumulus-oocyte complexes (COCs) for intracytoplasmic sperm injection (ICSI) can be time-consuming and may cause damage.
  • There is a need for innovative, efficient, and safe techniques to improve assisted reproductive technologies.

Purpose of the Study:

  • To introduce and evaluate acoustohydrodynamic tweezers (AHTs) as a novel noncontact method for COC denudation prior to ICSI.
  • To compare the efficacy and safety of AHTs with conventional manual pipetting (MP) denudation in a mouse model.

Main Methods:

  • AHTs were designed and fabricated based on focalized acoustic waves for contactless oocyte denudation.
  • Experiments involved comparing AHTs with MP using a mouse model, assessing cumulus removal efficiency, fertilization, embryo development, and live birth rates.
  • Safety was ensured by measuring acoustic intensity and thermal variability; lower hyaluronidase concentrations were used with AHTs.

Main Results:

  • AHTs achieved facile noncontact denudation without oocyte damage, reducing procedural time by 46% compared to MP.
  • Oocytes processed with AHTs showed comparable survival, fertilization, blastocyst rates, and embryo morphokinetics to those processed with MP.
  • Live birth rates were similar between AHT and MP groups (55.4% vs. 51.3%), indicating no adverse effects from the sound waves.

Conclusions:

  • Acoustohydrodynamic tweezers provide an expedited, safe, and reliable noncontact method for COC denudation for ICSI.
  • The comparable embryo development outcomes validate the safety and potential of AHTs for integration into ICSI-on-chip devices.