Development of multifunctional immobilized bacterial agents for multi-pesticides degradation and environment...

The proliferation of weeds, pests, and plant diseases in crop cultivation has driven the increased application of herbicide lactofen, insecticide acetamiprid, and fungicide carbendazim, contributing to environmental pollution. Microorganisms are requently employed to remove pesticide residues from the environment. However, Liquid bacterial agents encounter difficulties in transportation and preservation during application and the current immobilized bacterial agents have a single degradation function. This study developed immobilized bacterial agents containing the lactofen-degrading strain Bacillus sp. Za, the acetamiprid-degrading strain Pigmentiphaga sp. D-2, and the carbendazim-degrading strain Rhodococcus sp. djl-6. Preparation conditions, including activated carbon concentration, sodium alginate (SA), CaCl₂, and immobilization time, were optimized using the response surface method (RSM). The degradation performance of the immobilized bacteria was evaluated, with degradation rates exceeding 70% for all three pesticides under conditions of 30 °C, pH 7.0, and 6% inoculation over 48 h. The immobilized bacterial agents were stored at pH 7.0 and 4 °C for 180 days, maintaining a preservation rate of 51.26% with a viable cell count of 1.04 × 10⁸ CFU/g. These agents effectively remediated soil and water contaminated with multi-pesticides, achieving degradation rates of 92.50% and 98.50% for lactofen, 91.05% and 99.89% for acetamiprid, 88.43% and 98.99% for carbendazim within 21 in soil and 7 days in water, respectively. This study provides essential technical support for developing microbial agents capable of degrading multi-pesticides residues, with significant potential applications in agriculture and environmental protection.