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Related Experiment Videos

Coding sequence of coronavirus MHV-JHM mRNA 4.

M A Skinner, S G Siddell

    The Journal of General Virology
    |March 1, 1985
    PubMed
    Summary

    Researchers identified a novel coding sequence in the mouse hepatitis virus (MHV-JHM) mRNA 4. This sequence potentially encodes a 15.2 kDa protein, offering new insights into coronavirus replication.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Genomics

    Background:

    • The mouse hepatitis virus (MHV-JHM) is a significant model coronavirus.
    • Understanding viral gene expression is crucial for developing antiviral strategies.

    Purpose of the Study:

    • To determine the coding sequence at the 5' end of MHV-JHM mRNA 4.
    • To characterize the potential translation product of this mRNA.

    Main Methods:

    • M13/chain-terminator sequencing of cloned complementary DNA (cDNA).
    • Bioinformatic analysis to identify open reading frames (ORFs) and predict protein characteristics.

    Main Results:

    • A 417-base open reading frame (ORF) was identified in mRNA 4.
    • This ORF has the potential to encode a 15,200 molecular weight (139-residue) polypeptide.
    • The 3' end of this ORF overlapped with the start of an ORF in mRNA 5.
    • The predicted translation product is a basic polypeptide rich in threonine, with a hydrophobic N-terminus and basic C-terminus.

    Conclusions:

    • The identified coding sequence in MHV-JHM mRNA 4 likely represents a viral gene.
    • The predicted protein characteristics align with a previously described 14,000-14,500 molecular weight intracellular viral polypeptide.
    • This finding contributes to the understanding of the MHV-JHM genome organization and protein expression.

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