Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two acidic proteins associated with brain development in chick embryo.

T Shirao, K Obata

    Journal of Neurochemistry
    |April 1, 1985
    PubMed
    Summary

    This study analyzed chick optic tectum protein changes during development using gel electrophoresis. Two novel embryonic proteins, S5 and S6, were identified and found to be structurally homologous.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Relationships between squamous cell carcinoma antigen and cytokeratin 19 fragment values and renal function in oral cancer patients.

    International journal of oral and maxillofacial surgery·2022
    Same author

    Short communication: Effects of feeding purple corn (Zea mays L.) silage on productivity and blood superoxide dismutase concentration in lactating cows.

    Journal of dairy science·2019
    Same author

    Drebrin A regulates hippocampal LTP and hippocampus-dependent fear learning in adult mice.

    Neuroscience·2016
    Same author

    Allopregnanolone increases mature excitatory synapses along dendrites via protein kinase A signaling.

    Neuroscience·2015
    Same author

    Developmental α₂-adrenergic regulation of noradrenergic synaptic facilitation at cerebellar GABAergic synapses.

    Neuroscience·2013
    Same author

    Early definitive surgery for total colon aganglionosis: Duhamel procedure with right colon patch graft using GIA stapler.

    Pediatric surgery international·2013

    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Proteomics

    Background:

    • The chick optic tectum undergoes significant protein composition changes during development.
    • Understanding these changes is crucial for comprehending brain development.

    Purpose of the Study:

    • To analyze developmental changes in chick optic tectum protein composition.
    • To identify and characterize novel proteins appearing during embryonic development.

    Main Methods:

    • Two-dimensional gel electrophoresis (2D-PAGE) for protein separation and analysis.
    • Protein purification using precipitation and chromatography techniques.
    • Peptide mapping with V8 protease to assess structural homology.

    Main Results:

    • 54 major proteins were identified, with eight showing significant developmental abundance changes.
    • Two unique embryonic proteins, S5 (95 kDa, pI 4.5) and S6 (100 kDa, pI 4.5), were discovered.
    • S5 and S6 showed high structural homology, indicated by shared peptides from V8 protease mapping.
    • These proteins were found in the embryonic brain but not the embryonic liver.

    Conclusions:

    • The chick optic tectum exhibits dynamic protein expression during development.
    • Proteins S5 and S6 represent novel developmentally regulated proteins in the avian brain.
    • The structural similarity between S5 and S6 suggests a shared functional role during embryonic development.

    Related Experiment Videos