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Updated: May 7, 2025
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
Construction of AMPK-related circRNA network in mouse myocardial ischemia-reperfusion injury model
Yang Song1, Yi Zhao1, Xiaodi Zhang1
1Department of Cardiovascular Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563000, China.
Objective:
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) in MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Methods:
The mouse MIRI model was constructed by ligation of the left anterior descending artery. After the model was successfully established, the related indicators of cardiac function were detected, and high-throughput sequencing was performed on the myocardial tissue of the mice.
Results:
MIRI model was successfully constructed, and two AMPK related differentially expressed loops (novel_circ_043550 and novel_circ_035243) were screened out. A circRNA-miRNA-mRNA network consisting of 2 circRNA, 28 microRNA(miRNA) and 229 messengerRNA (mRNA) was constructed.
Conclusions:
This study reveals the differential expression of several AMPK-related circRNAs in MIRI in mice, and the AMPK-related circRNA regulatory network is constructed, suggesting that AMPK-related circRNA may have potential clinical application prospects as a potential molecular marker and therapeutic target for MIRI.
Insights
Researchers identified novel circular RNAs (circRNAs) linked to adenosine monophosphate-activated protein kinase (AMPK) in a mouse model of myocardial ischemia-reperfusion injury (MIRI). This discovery offers potential new molecular markers and therapeutic targets for MIRI.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Genomics
Background:
- Myocardial ischemia-reperfusion injury (MIRI) is a significant clinical challenge.
- Adenosine monophosphate-activated protein kinase (AMPK) plays a crucial role in cellular energy homeostasis and has been implicated in cardiovascular protection.
- Circular RNAs (circRNAs) are emerging as key regulators in various biological processes, including cardiovascular diseases.
Purpose of the Study:
- To screen for differentially expressed AMPK-related circRNAs in a mouse model of MIRI.
- To construct a regulatory network of AMPK-related circRNAs, microRNAs (miRNAs), and messenger RNAs (mRNAs) in MIRI.
- To identify potential molecular markers and therapeutic targets for MIRI.
Main Methods:
- A mouse model of MIRI was established by ligating the left anterior descending artery.
- Cardiac function indicators were assessed post-MIRI induction.
- High-throughput sequencing was employed to analyze myocardial tissue for circRNA expression.
Main Results:
- The MIRI mouse model was successfully constructed.
- Two novel AMPK-related differentially expressed circRNAs (novel_circ_043550 and novel_circ_035243) were identified.
- A complex circRNA-miRNA-mRNA regulatory network involving 2 circRNAs, 28 miRNAs, and 229 mRNAs was constructed.
Conclusions:
- This study identified differentially expressed AMPK-related circRNAs in MIRI.
- The constructed AMPK-related circRNA regulatory network provides insights into MIRI pathogenesis.
- AMPK-related circRNAs show potential as molecular markers and therapeutic targets for MIRI.
