Related Experiment Video
Updated: May 7, 2025
07:04
Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
Published on: January 24, 2025
257
Newcastle Disease Vaccination Program in Broilers Using an Apathogenic Heat-Resistant Vaccine.
S A Ghafouri1, A Ghaniei1, M Moghani1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Archives of Razi Institute
|December 31, 2024
Summary
The heat-resistant Newcastle disease virus (NDV4HR) vaccine effectively protected chickens against virulent Newcastle disease (ND) challenge, showing no virus shedding and good weight gain. This thermotolerant vaccine offers a promising solution for ND control in poultry.
Area of Science:
- Veterinary Virology
- Poultry Health
- Immunology
Background:
- Newcastle disease (ND) is a significant global threat to poultry production.
- Thermostable Newcastle disease virus (NDV) vaccines are crucial for maintaining flock immunity, especially in regions with limited cold chain infrastructure.
- The NDV4HR vaccine is a live, apathogenic, heat-resistant strain designed for enhanced stability and efficacy.
Purpose of the Study:
- To evaluate the efficacy of the NDV4HR vaccine in broiler chickens against a virulent Newcastle disease virus (NDV) challenge.
- To compare the immune response, clinical signs, viral shedding, and weight gain in chickens vaccinated with NDV4HR versus conventional vaccination programs or no vaccination.
- To assess the protective potential of a thermostable NDV vaccine under challenging conditions.
Main Methods:
- Sixty one-day-old broilers were divided into three groups: conventional vaccination (Group A), no vaccination (Group B), and NDV4HR vaccination (Group C).
- Group C received the NDV4HR vaccine orally six times from day 1 to day 35.
- All groups were challenged with virulent NDV (genotype VIId) on day 35, and outcomes including antibody titers, clinical signs, necropsy, weight gain, and viral shedding were monitored.
Main Results:
- The NDV4HR vaccine (Group C) demonstrated no viral shedding post-challenge, unlike Groups A and B.
- Group C exhibited the highest weight gain (2204±106g) compared to other groups.
- While antibody titers were highest in the unvaccinated group (Group B), Groups C and A showed similar clinical outcomes and necropsy findings, with significantly lower morbidity and mortality in vaccinated groups compared to the unvaccinated group.
Conclusions:
- The NDV4HR vaccine successfully induced an immune response that protected chickens against virulent NDV challenge.
- The thermostable nature and efficacy of the NDV4HR vaccine make it a valuable tool for controlling Newcastle disease, particularly in resource-limited settings.
- No viral shedding in the NDV4HR vaccinated group indicates reduced transmission potential, enhancing its epidemiological benefits.
