Related Experiment Video
Updated: Jun 4, 2025
In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Multitarget Peptides Released by In Vitro Static Gastrointestinal Digestion of an Amaranth Protein Beverage
S E Suárez1,2,3, A Quiroga1,2, A C Sabbione1,2
1Laboratorio de Investigación, Desarrollo e Innovación de Proteínas Alimentarias (LIDiPA), Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA, CONICET-CIC-UNLP), Street 47 and 116, La Plata, Buenos Aires, Argentina.
Amaranth protein beverages release beneficial peptides during simulated digestion, enhancing health properties. This study reveals amaranth as a promising source of functional peptides for improved nutrition and well-being.
Area of Science:
- Food Science
- Nutritional Biochemistry
- Protein Chemistry
Background:
- Growing interest in alternative proteins like amaranth due to dietary shifts and environmental concerns.
- Amaranth protein beverages are emerging as a sustainable and nutritious option.
- Understanding the bioaccessibility and bioactivity of amaranth peptides is crucial.
Purpose of the Study:
- To assess the release and bioactivity of peptides from amaranth protein beverages after simulated gastrointestinal digestion.
- To evaluate the antihypertensive, antioxidant, and antithrombotic potential of amaranth-derived peptides.
- To identify and characterize the bioactive peptides generated.
Main Methods:
- Static simulated gastrointestinal digestion (SGD) protocol applied to amaranth protein beverage.
- In vitro assays to measure ACE inhibition, antioxidant capacity (ABTS+, AAPH), and antithrombotic activity.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for peptide identification.
Main Results:
- SGD significantly increased the release of bioactive peptides from amaranth protein.
- Enhanced ACE inhibition, antioxidant activity against radicals, and antithrombotic effects were observed.
- LC-MS/MS identified 31 peptides (9-21 amino acids) linked to various bioactivities.
Conclusions:
- Amaranth protein beverages are a rich source of bioactive peptides with significant health-promoting properties.
- Simulated digestion enhances the functional potential of amaranth peptides.
- Further research is warranted to isolate and characterize specific peptides for therapeutic applications.
