First microsatellite markers for the Amazonian species Copaifera multijuga Hayne developed using next-generation
Rayssa Gomes Vasconcelos1, Sandra Marcela Hernández-Rangel2, Izeni Pires Farias2
1Programa de Pós-Graduação em Ciências de Florestas Tropicais (PPG-CFT), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Amazonas, Brazil. rayssa.gomesvasc@gmail.com.
Researchers developed novel microsatellite markers for Copaifera multijuga, a valuable Amazonian tree. These genetic tools revealed moderate to high genetic diversity in a native population, aiding conservation efforts.
Area of Science:
- Botany
- Genetics
- Conservation Biology
Background:
- Copaifera multijuga, an Amazonian tree, is exploited for oleoresin.
- Limited research exists on the genetic characterization of native populations.
Purpose of the Study:
- Develop novel microsatellite markers for Copaifera multijuga.
- Characterize the genetic diversity of a native population.
Main Methods:
- Utilized next-generation sequencing (Ion Torrent PGM™).
- Designed and validated 19 microsatellite primer pairs.
- Analyzed genetic diversity using 13 polymorphic loci in 47 trees.
Main Results:
- Developed 13 efficient microsatellite markers.
- Observed heterozygosity (Ho) of 0.67 and expected heterozygosity (He) of 0.64.
- Found an average of 5.4 alleles per locus, with most loci being informative.
Conclusions:
- The developed microsatellites effectively characterize Copaifera multijuga genetic diversity.
- These markers are valuable for future conservation strategies.
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