Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

13.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fc-fucosylation of Plasmodium falciparum-specific IgG varies with antigen and immunization.

The Journal of infectious diseases·2026
Same author

Malaria parasites undergo a rapid and extensive metamorphosis after invasion of the host erythrocyte.

EMBO reports·2025
Same author

Structural Plasticity of Plasmodium falciparum Plasmepsin X to Accommodate Binding of Potent Macrocyclic Hydroxyethylamine Inhibitors.

Journal of molecular biology·2025
Same author

<i>Plasmodium falciparum</i> protein phosphatase PP7 is required for early ring-stage development.

mBio·2024
Same author

Mixed alkyl/aryl phosphonates identify metabolic serine hydrolases as antimalarial targets.

Cell chemical biology·2024
Same author

Peptidic Boronic Acid <i>Plasmodium falciparum</i> SUB1 Inhibitors with Improved Selectivity over Human Proteasome.

Journal of medicinal chemistry·2024

Related Experiment Video

Updated: Jun 4, 2025

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases
09:13

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

1.3K

A scaleable inducible knockout system for studying essential gene function in the malaria parasite.

Abhinay Ramaprasad1, Michael J Blackman1,2

  • 1Malaria Biochemistry Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT London, UK.

Nucleic Acids Research
|December 31, 2024
PubMed
Summary

Researchers developed SHIFTiKO, a scalable gene editing tool for malaria parasites. This system enables large-scale studies of essential genes, aiding in understanding parasite propagation and identifying new drug targets.

More Related Videos

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
09:25

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

Published on: September 18, 2018

9.9K
Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages
08:23

Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Published on: May 30, 2019

11.5K

Related Experiment Videos

Last Updated: Jun 4, 2025

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases
09:13

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

1.3K
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
09:25

CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

Published on: September 18, 2018

9.9K
Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages
08:23

Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Published on: May 30, 2019

11.5K

Area of Science:

  • Genetics
  • Parasitology
  • Molecular Biology

Background:

  • Malaria parasites require numerous genes for survival within red blood cells.
  • Studying essential gene functions necessitates inducible gene expression interference systems.
  • Current DiCre-lox systems lack scalability for simultaneous analysis of multiple genes.

Purpose of the Study:

  • To develop a scalable strategy for studying essential gene functions in malaria parasites.
  • To overcome limitations of existing DiCre-lox systems for high-throughput genetic screening.
  • To enable inducible phenotypic screening of multiple genes concurrently.

Main Methods:

  • Developed SHIFTiKO (frameshift-based trackable inducible knockout), a novel scalable strategy.
  • Utilized short, barcoded repair templates to insert loxP sites around target gene regions.
  • Induced DiCre-mediated excision causing frameshift mutations for genetic ablation.
  • Employed dual DNA barcodes for verification and collective phenotyping.

Main Results:

  • Successfully applied SHIFTiKO to screen malarial rhomboid proteases.
  • Identified blood stage-specific essentiality of screened rhomboid proteases.
  • Demonstrated SHIFTiKO's capability for assessing growth fitness and specific impairments.

Conclusions:

  • SHIFTiKO provides a powerful, scalable platform for inducible phenotypic screens in malaria parasites.
  • Facilitates large-scale study of essential gene functions, crucial for understanding parasite biology.
  • Aids in identifying critical genes for parasite propagation and potential therapeutic targets.