Related Experiment Video

Updated: Aug 7, 2026

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Inhibitory effect of interferon on the genetic and oncogenic transformation by viral and cellular genes

M Perucho, M Esteban

    Journal of Virology
    |April 1, 1985
    PubMed

    Insights

    Interferon treatment significantly inhibits gene transfer and oncogenic transformation in rodent cell lines. This suggests interferon affects the stabilization or integration of foreign DNA, impacting gene expression.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Virology

    Background:

    • Rodent cell lines (LTk- and NIH 3T3) are standard models for gene transfer studies.
    • Interferon is known to modulate cellular responses to viral infections and transformation.

    Purpose of the Study:

    • To investigate the effect of interferon on genetic and oncogenic transformation.
    • To determine how interferon influences gene transfer mediated by various viral and cellular oncogenes.

    Main Methods:

    • Gene transfer experiments using LTk- and NIH 3T3 cells.
    • Treatment with interferon prior to or during transformation.
    • Analysis of stable transformation by genes including chicken thymidine kinase (tk), Ecogpt, simian virus 40, v-Ha-ras, and human c-Ha-ras/c-Ki-ras oncogenes.

    Main Results:

    • Interferon severely inhibited stable transformation in both cell lines.
    • The inhibitory effect was consistent across different oncogenes and viral DNA.
    • Interferon appears to impede the stabilization or integration of exogenous DNA.

    Conclusions:

    • Interferon treatment significantly impairs gene transfer and oncogenic transformation.
    • The mechanism likely involves interference with DNA stabilization or integration processes.
    • Interferon may also influence the expression of transferred genes.

    More Related Videos

    High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes10:00

    High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

    Published on: March 24, 2015

    A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF311:44

    A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

    Published on: January 24, 2016

    Related Concept Videos

    Eukaryotic Transcription Inhibitors01:52

    Eukaryotic Transcription Inhibitors

    Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
    Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
    Mechanisms of Retrovirus-induced Cancers01:51

    Mechanisms of Retrovirus-induced Cancers

    Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
    Immune Response Against Viral Pathogens01:29

    Immune Response Against Viral Pathogens

    The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
    NK Cells
    NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
    Inhibitors of Viral Protein Synthesis01:30

    Inhibitors of Viral Protein Synthesis

    Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
    Inhibitors Of Virion Release01:25

    Inhibitors Of Virion Release

    Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
    Inhibitors of Virion Maturation and Assembly01:19

    Inhibitors of Virion Maturation and Assembly

    As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...