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Lysozyme/Tracheal Antimicrobial Peptide-Based Tissue-Specific Expression Antimicrobial Plasmids Show Broad-Spectrum

Yi Yang1,2, Yining Meng1, Daijie Chen3

  • 1Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.

Advanced Biology
|December 31, 2024
PubMed

Insights

Lysozyme (LYZ)/tracheal antimicrobial peptide (TAP)-based tissue-specific expression antimicrobial plasmids (TSEAP) show broad-spectrum antibacterial activity. These novel non-antibiotic treatments effectively reduced mastitis symptoms in mice and were found to be safe.

Area of Science:

  • Microbiology
  • Biotechnology
  • Veterinary Medicine

Background:

  • Antibiotic resistance is a significant global health threat, necessitating alternative treatments for bacterial infections.
  • Mastitis, a common bacterial disease, requires effective and safe therapeutic strategies.

Purpose of the Study:

  • To evaluate the efficacy and safety of lysozyme (LYZ)/tracheal antimicrobial peptide (TAP)-based tissue-specific expression antimicrobial plasmids (TSEAP) for mastitis treatment.
  • To explore TSEAP as a potential non-antibiotic alternative for bacterial diseases.

Main Methods:

  • TSEAP constructs incorporating LYZ and TAP were designed and tested in a mouse model of mastitis.
  • Infection was induced using Staphylococcus sciuri, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa.
  • Safety was assessed through behavioral tests, weight monitoring, blood biochemistry, and organ coefficient analysis.

Main Results:

  • LYZ/TAP-based TSEAP significantly reduced clinical mastitis symptoms caused by the tested bacterial pathogens.
  • Comprehensive safety evaluations, including behavioral, physiological, and biochemical parameters, indicated no adverse effects from LYZ/TAP-based TSEAP transfection.
  • The study demonstrated the broad-spectrum antibacterial activity of LYZ/TAP-based TSEAP.

Conclusions:

  • LYZ/TAP-based TSEAP represent a promising non-antibiotic therapeutic approach for bacterial diseases, including mastitis.
  • These findings offer new insights into developing safe and effective antimicrobial strategies to combat antibiotic resistance.
Keywords:
broad‐spectrum antibacterial activitylysozymemastitis modeltissue‐specific expression antimicrobial plasmidtracheal antimicrobial peptide

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