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Updated: Aug 18, 2026

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
Published on: April 8, 2015
Variations in the cell cycle of slow-growing Escherichia coli 15T-
Abstract:
DNA synthesis was measured via 3H-thymidine pulses, and patterns of incorporation were compared to cell number increases during synchronous division of Escherichia coli 15T- (555-7) in M9.01% aspartate. The timing of chromosome initiation, duration of chromosome synthesis, and the D period were found to vary over the three cycles of division analysed. In addition, the D period measured by comparing 3H-thymidine incorporation and concomitant division, differed from D period measurements made by timing division cessation in DNA synthesis inhibitors.
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