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Updated: Jul 10, 2026
Analysis of Cell Migration within a Three-dimensional Collagen Matrix
Published on: October 5, 2014
Impact of Rhenium(I)-Diselenoether Compound on Cell Adhesion, Migration, Invasion Capacities, and MMP-2 Release in
Philippe Collery1, Didier Desmaele2, Adhikesavan Harikrishnan3
1Société de Coordination de Recherches Thérapeutiques, Algajola, France; philippe.collery@gmail.com.
Background/Aim:
Organometallic complexes can decrease adhesion, migration, invasion of cancer cells, mainly through regulation of the extracellular matrix and therefore act against metastases. The aim was to investigate the anti-invasive properties of a rhenium-based metal compound, rhenium(I)-diselenoether (Re-diSe) and its effects on matrix metalloproteinase MMP-2, a key player in metastatic processes, in cultured MDA-MB231 triple-negative breast cancer cells.
Materials And Methods:
Matrigel was utilized to assess cancer cell adhesion to the extracellular matrix. Migration capacity was evaluated through the wound healing assay, while invasion assays were conducted using transwell invasion assay by the crystal violet dye. MMP-2 levels were quantified in the cell medium using a fluorometric kit assay. Cells were exposed to increasing concentrations of the drug (5, 10, 20, 50 and 100 μM). Three time points of drug exposure on the cells were investigated (48, 96 and 120 h).
Results:
Re-diSe exhibited effectiveness even at doses as low as 5 or 10 μM in all biological assays. The time of exposure of 120 h significantly improved the effect on the invasion capacity of the cancer cells by comparison with 48 and 96 h.
Conclusion:
The results highlight the anti-invasive properties of Re-diSe and its impact on MMP-2 levels in MDA-MB231 breast cancer cells.
Insights
Rhenium(I)-diselenoether (Re-diSe) effectively inhibits triple-negative breast cancer cell invasion and migration by reducing matrix metalloproteinase MMP-2. This metal compound shows promise in combating cancer metastasis.
Area of Science:
- Organometallic chemistry
- Cancer biology
- Drug discovery
Background:
- Organometallic complexes can inhibit cancer cell metastasis by regulating the extracellular matrix.
- Metastasis involves cancer cell adhesion, migration, and invasion, processes often mediated by matrix metalloproteinases (MMPs).
Purpose of the Study:
- To investigate the anti-invasive effects of a novel rhenium(I)-diselenoether (Re-diSe) compound.
- To determine the impact of Re-diSe on matrix metalloproteinase MMP-2 in MDA-MB231 triple-negative breast cancer cells.
Main Methods:
- Cancer cell adhesion was assessed using Matrigel.
- Cell migration was evaluated via wound healing assays.
- Invasion assays and MMP-2 quantification were performed using transwell assays and fluorometric kits, respectively.
- Cells were treated with Re-diSe (5–100 μM) at various time points (48, 96, 120 h).
Main Results:
- Re-diSe demonstrated efficacy at low concentrations (5–10 μM) across all tested assays.
- A 120-hour exposure significantly enhanced the anti-invasive effect compared to shorter durations (48 and 96 h).
Conclusions:
- Re-diSe exhibits significant anti-invasive properties against triple-negative breast cancer cells.
- The compound effectively modulates MMP-2 levels, suggesting a mechanism for inhibiting cancer metastasis.
