Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A longitudinal examination of gut microbiota dysbiosis and neuro-behavioral phenotypes of poly(I:C)-maternal immune activation in a preclinical model of autism and schizophrenia.

Brain, behavior, and immunity·2026

Same author

Gut Microbiota Differences in Down Syndrome Are Most Pronounced in Childhood and Diminish With Age.

International journal of microbiology·2026

Same author

Soluble Fiber Intake Restores the Estrous Cycle and Sex Hormone Alterations in APP/PS1 Female Mice.

Cellular and molecular neurobiology·2026

Same author

MicroRNA Quantification on Fast and Slow-Twitch Skeletal Muscles of Mouse.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Gene Expression Analysis on Single Myofibers from Mouse Edl and Soleus Muscles.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

The flavanol (-)-epicatechin induced different histological features and myogenic protein levels in triceps, gastrocnemius and plantaris of δ-sarcoglycan-deficient mice.

Tissue & cell·2026

Search research articles

Related Experiment Video

Updated: Jun 4, 2025

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Changes in miRNA Pattern Expression Associated With COVID-19 Severity.

Paola B Zárate-Segura¹, Macario Martínez-Castillo², Aarón Paris Garduño-Gutiérrez³

¹Laboratorio de Medicina Traslacional, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico; pzarates@ipn.mx.

In Vivo (Athens, Greece)

|December 31, 2024

Summary

Specific microRNAs (miRNAs) are over-expressed in severe COVID-19 patients. These miRNAs, including miR-490-3p, may serve as potential biomarkers for predicting the clinical course of coronavirus disease 2019.

Keywords:

COVID-19 miR-195-5p miR-431-5p miR-454-3p miR-490-3p severity

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Related Experiment Videos

Last Updated: Jun 4, 2025

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Area of Science:

Molecular Biology
Virology
Genetics

Background:

Coronavirus disease 2019 (COVID-19) presents diverse clinical symptoms.
Host microRNAs (miRNAs) can influence COVID-19 progression and complications.
Understanding host factors like miRNA expression is crucial for managing severe SARS-CoV-2 infection.

Purpose of the Study:

To investigate the expression patterns of endogenous miRNAs in severe COVID-19 patients.
To compare miRNA expression between 80 severe COVID-19 cases and healthy controls.
To identify specific miRNAs associated with severe SARS-CoV-2 infection.

Main Methods:

miRNA expression screening using TaqMan Low-Density Array.
Validation of specific miRNA expression (miR-490-3p, miR-195-5p, miR-454-3p, miR-431-5p) via RT-qPCR.
In silico analysis for target identification and pathway prediction.

Main Results:

Four miRNAs (miR-490-3p, miR-195-5p, miR-454-3p, miR-431-5p) were significantly over-expressed in severe COVID-19 patients compared to controls.
miR-490-3p showed over-expression in both male and female COVID-19 patients.
These findings highlight specific miRNA dysregulation in severe SARS-CoV-2 infection.

Conclusions:

Specific miRNAs demonstrate altered expression in severe COVID-19.
These over-expressed miRNAs may function as potential biomarkers for predicting COVID-19 clinical outcomes.
Further research into miRNA roles could inform therapeutic strategies for severe coronavirus disease 2019.