Related Experiment Video
Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Novel, Rapid, and Simple Isocratic UPLC-UV Method for Estimating Nitrate and Nitrite Contents in Environmental Water
Pradeep Kumar Gollapudi1, Kranthi Kumar Gollapudi1, Dr Nimmagadda Padmaja2
1Dr Reddy's Laboratories, IPDO, API AR&D Bachupally, Hyderabad 500090, India.
Abstract:
In this study, we reported a novel reverse-phase UPLC method for the simultaneous estimation of nitrite and nitrate ions using a Quality by Design (QbD) approach. Nitrite and nitrate ions were separated on an ACQUITY CSH Fluoro-Phenyl column (100 × 2.1 mm, 1.7 μm) in isocratic mode with a mobile phase composition of solvent A and solvent B in a ratio of 980:20 v/v. Solvent A consisted of 100% Milli-Q water, while solvent B comprised 0.5 mL of formic acid in 1000 mL of methanol. The column temperature was maintained at 40 °C, and the flow rate was 0.6 mL/min. Detection was carried out at 214 nm with an injection volume of 5.0 μL. The method was developed and validated in accordance with ICH Q2 (R2) guidelines Validation parameters, including system suitability, sensitivity, precision, linearity, and accuracy, were within acceptable ranges. These findings suggest that the developed RP-UPLC method is highly sensitive and suitable for the estimation of nitrite and nitrate ions in various environmental water samples.
More Related Videos
07:14Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
Published on: December 20, 2016
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Related Concept Videos
Voltammetry: Stripping Methods
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Gravimetry: Overview
In precipitation gravimetry, the analyte is converted into a precipitate and weighed. For example, the silver content in a sample can be estimated by precipitating and...
Precipitation and Co-precipitation
High-Performance Liquid Chromatography: Types of Detectors
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...