Related Experiment Video
Updated: May 7, 2025
Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model
Published on: April 18, 2025
Morin Ameliorates Myocardial Injury in Diabetic Rats via Modulation of Autophagy, Apoptosis, Inflammation, and
1Department of Endocrinology, Huaihe Hospital of Henan University, Kaifeng, 475000, People's Republic of China.
Background:
Morin is a flavonol with beneficial effects on diabetic-related injuries. However, the effect of morin on diabetic cardiomyopathy and its association with autophagy, apoptosis, inflammation, and oxidative stress remains unclear. The current study aimed to reveal the mechanisms underlying morin-mediated protection against cardiac failure in diabetic rats.
Methods:
Diabetic cardiomyopathy in albino Wistar rats was induced by streptozotocin (STZ). After treatment with a dose of 25, 50, and 100 mg/kg/day orally for the next 60 days, autophagic (p62, LC3, and BECN1), apoptotic (BCL2, CASP-3, and CASP9), inflammatory (IL-1β, IL-6, TNF-α), and oxidative stress (CAT, SOD, and MDA) markers in protein and gene levels as well as cardiac function tests were measured.
Results:
The findings revealed that long-term morin treatment improved weight gain, lipid and glycemic profile, hypertension, and cardiac hypertrophy and fibrosis in diabetic rats compared to controls (p-value<0.001). Moreover, the upregulation of BCL-2, LC3, and BECN1 along with the downregulation of p62, CASP-3, and CASP-9 revealed that morin suppressed apoptosis and promoted autophagy in the cardiac tissue of rats with diabetes (p-value<0.05). Additionally, the reduction in IL-1β, IL-6, TNF-α, and MDA levels and the increment of SOD and CAT activity suggested that morin decreased inflammation and apoptosis in the heart of the rat models of diabetes (p-value<0.01).
Conclusion:
These results may highlight the potential properties of morin as a therapeutic strategy for diabetic cardiomyopathy.
Insights
Morin treatment improved cardiac function in diabetic rats by reducing inflammation, oxidative stress, and apoptosis while promoting autophagy. This highlights morin
Area of Science:
- Cardiovascular Research
- Metabolic Disorders
- Pharmacology
Background:
- Diabetic cardiomyopathy is a significant complication of diabetes.
- The protective mechanisms of morin against diabetic cardiomyopathy are not fully understood.
- Morin is a flavonol with potential therapeutic benefits.
Purpose of the Study:
- To investigate the effects of morin on diabetic cardiomyopathy in rats.
- To elucidate the underlying mechanisms involving autophagy, apoptosis, inflammation, and oxidative stress.
Main Methods:
- Diabetic cardiomyopathy was induced in Wistar rats using streptozotocin (STZ).
- Rats were treated with varying doses of morin (25, 50, 100 mg/kg/day) for 60 days.
- Cardiac function, autophagic markers (p62, LC3, BECN1), apoptotic markers (BCL2, CASP-3, CASP9), inflammatory markers (IL-1β, IL-6, TNF-α), and oxidative stress markers (CAT, SOD, MDA) were assessed.
Main Results:
- Morin treatment improved glycemic control, lipid profile, hypertension, cardiac hypertrophy, and fibrosis.
- Morin suppressed apoptosis and promoted autophagy in cardiac tissue by modulating key protein expressions.
- Morin reduced inflammation and oxidative stress markers in the hearts of diabetic rats.
Conclusions:
- Morin demonstrates significant cardioprotective effects in a rat model of diabetic cardiomyopathy.
- Morin exerts its benefits by modulating autophagy, apoptosis, inflammation, and oxidative stress pathways.
- Morin holds promise as a potential therapeutic agent for managing diabetic cardiomyopathy.
