Related Experiment Video
Updated: May 7, 2025
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
[Study on the Production of Anti-D and Anti-E Mixed Antibodies by Alloimmunization in RhD Variant Type33 Recipients]
1Blood Group Reference Laboratory, Ningxia Blood Center, Yinchuan 750000, Ningxia Hui Autonomous Region, China.
Objective:
To investigate the cause of the production of anti-D and anti-E mixed antibody in an RhD positive patient.
Methods:
The ABO/Rh blood group typing and irregular antibody specificity were identified by conventional serological methods, the RHD gene exon 1-10 and heterozygous analysis were performed by sequence-specific primer polymerase chain reaction (PCR-SSP), and the whole exon sequence was analyzed by first-generation sequencing.
Results:
The patient's Rh blood group was weak D Type33, with the allele was RHD*01W.33, the patients was found to be RhD heterozygous, with an Rh typing of Ccee, and the patient had developed anti-D combined with anti-E mixed antibodies.
Conclusion:
The patient has A c.520G>A mutation in exon 4 of the RHD gene, to lead decreased expression of RhD antigen in red blood cells and the anti-D and anti-E mixed antibodies were produced by transfusion immunostimulation.
More Related Videos
18:48In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Published on: August 12, 2017
11:31Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Published on: January 2, 2013
Related Concept Videos
01:10Blood Typing
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...
02:45Blood Transfusion and Agglutination
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
01:19Rh Blood Group
02:20Blood Types
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...
00:42Cross-reactivity
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...