Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

305
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
305
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

295
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
295

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoscale morphological and structural analysis of round and donut oligomers formed by C-terminal domain of TDP-43.

Physical chemistry chemical physics : PCCP·2026
Same author

Single Amino Acid-Based Control of Fibril Supramolecular Chirality.

The journal of physical chemistry letters·2026
Same author

Non-Mendelian inheritance of DNA methylation patterns in mice.

Nature genetics·2026
Same author

In search of a mouse model of Spaceflight Associated Neuro-ocular Syndrome using one-carbon genetics.

Scientific reports·2026
Same author

Formation of S- and Z-twist supramolecular micro-ropes by peptide stereoisomers.

Nature communications·2026
Same author

Astaxanthin, meclizine, mitoglitazone, pioglitazone, alpha-ketoglutarate, mifepristone, methotrexate, and atorvastatin-telmisartan do not increase lifespan in UM-HET3 mice.

GeroScience·2026
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K

Noninvasive Optical Sensing of Aging and Diet Preferences Using Raman Spectroscopy.

Isaac D Juárez1, Alexandra Naron1, Heidi Blank1

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, United States.

Analytical Chemistry
|January 1, 2025
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy (RS) accurately assesses body composition changes due to diet, like Vegan, Typical American, and Ketogenic patterns. This noninvasive technique also detects folate deficiency, animal sex, and chronological age, enabling personalized nutritional guidance.

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K
Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

4.1K

Related Experiment Videos

Last Updated: Jun 4, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.0K
Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

4.1K

Area of Science:

  • Biomedical Engineering
  • Nutritional Science
  • Analytical Chemistry

Background:

  • Accurate dietary monitoring is crucial for understanding diet's impact on health and obesity.
  • Technological advancements are enabling personalized nutritional guidance.
  • Noninvasive methods are needed to track dietary exposures and their effects.

Purpose of the Study:

  • To explore Raman spectroscopy (RS) as a noninvasive method for assessing diet-induced changes.
  • To evaluate RS's accuracy in determining body composition based on habitual dietary patterns.
  • To investigate RS's potential for predicting nutritional deficiencies and physiological states.

Main Methods:

  • Utilized Raman spectroscopy (RS), a noninvasive and nondestructive analytical technique.
  • Applied RS to analyze changes in mice skin in response to controlled dietary exposures (Vegan, Typical American, Ketogenic).
  • Correlated RS data with body composition, folate levels, sex, and chronological age.

Main Results:

  • RS accurately determined body composition influenced by different dietary patterns.
  • RS showed high accuracy in predicting folate deficiency and identifying animal sex.
  • RS effectively tracked the chronological age of the mice.
  • The technique relies on changes in collagen vibrational bands.

Conclusions:

  • Raman spectroscopy is a highly accurate, noninvasive tool for monitoring diet-induced body composition changes.
  • RS has potential applications in personalized nutrition, health monitoring, and diagnostics.
  • The hand-held nature of the spectrometer suggests practical, real-world monitoring capabilities.