Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative analysis of collagen architecture in the human uterotubal junction (UTJ) using optical coherence tomography imaging (OCT).

Scientific reports·2026
Same author

Improved endoscope for imaging and cell collection in the fallopian tubes.

Biophotonics discovery·2026
Same author

Feasibility of non-imaging, random-sampling second harmonic generation measurements to distinguish colon cancer.

Biophotonics discovery·2026
Same author

Optical coherence tomography and elastography for <i>ex vivo</i> visualization of early gastric cancer.

Journal of biomedical optics·2026
Same author

Regulation of skeletal muscle development and metabolism in broiler chickens by Urolithin A through threonine kinase 1 pathway activation.

Poultry science·2026
Same author

Dietary urolithin a improves hepatic antioxidant function and laying performance in aging hens via Nrf2 signaling pathway activation.

Poultry science·2026
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

398

A domed window chamber for multi-modality optical imaging.

Photini Rice1, Makenna Aitken1, Hasina Shir1

  • 1Biomedical Engineering, The University of Arizona, Tucson, AZ, USA.

Biotechniques
|January 2, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel dorsal skin flap window chamber with a domed fluorinated ethylene propylene (FEP) window. This modification enables multimodal in vivo imaging using light sheet fluorescence microscopy (LSFM), optical coherence tomography (OCT), and multiphoton microscopy (MPM).

Keywords:
Dorsal skin flapfluorinated ethylene propylenelight sheet fluorescence microscopylight sheet imagingmulti-photon microscopyoptical coherence tomography

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
10:53

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Published on: January 20, 2022

3.2K

Related Experiment Videos

Last Updated: Jun 4, 2025

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

398
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K
Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
10:53

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Published on: January 20, 2022

3.2K

Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Optical Imaging

Background:

  • Dorsal skin flap window chambers are essential for in vivo microscopy.
  • Current chambers with flat glass windows limit light sheet fluorescence microscopy (LSFM) performance.
  • LSFM requires specific sample geometry and refractive index matching for optimal imaging.

Purpose of the Study:

  • To engineer a modified window chamber for enhanced multimodal in vivo imaging.
  • To adapt dorsal skin flap window chambers for light sheet fluorescence microscopy (LSFM).
  • To enable simultaneous optical coherence tomography (OCT), multiphoton microscopy (MPM), and LSFM.

Main Methods:

  • Designed a modified window chamber with a domed viewing window made of fluorinated ethylene propylene (FEP).
  • FEP material was chosen for its refractive index (n) matching properties similar to water and tissue.
  • Validated the chamber design through in vitro imaging of collagen gels and microsphere phantoms.
  • Demonstrated in vivo multimodality imaging using OCT, MPM, and LSFM on a custom mouse platform.

Main Results:

  • The domed FEP window showed minimal impact on signal strength and image resolution in vitro.
  • The modified chamber successfully accommodated LSFM requirements for sample positioning and refractive index.
  • Successful in vivo demonstration of simultaneous OCT, MPM, and LSFM imaging was achieved.

Conclusions:

  • The modified dorsal skin flap window chamber with a domed FEP window is compatible with LSFM, OCT, and MPM.
  • This innovation expands the capabilities of in vivo multimodal imaging in preclinical research.
  • The design facilitates advanced optical microscopy techniques for studying biological processes.