Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservation of Protein Domains Over Different Proteins

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clinical significance of high-density lipoprotein cholesterol and its dynamic change in patients with lymphoma-associated hemophagocytic lymphohistiocytosis.

Therapeutic advances in medical oncology·2026

Same author

The PABS system under the pandemic agreement: Current status, controversies in negotiations, and future trends.

Biosafety and health·2026

Same author

Oral microbiome modulation mitigates hyperglycemia exacerbation in gestational diabetes mellitus.

Nature communications·2026

Same author

Management of diffuse large B‑cell lymphoma in older adults: current strategies and emerging directions.

Therapeutic advances in medical oncology·2026

Same author

Selective autophagy fine-tunes plant immunity to promote cell survival during viral infection.

Science (New York, N.Y.)·2026

Same author

Ubiquitin-dependent mitochondrial protein degradation ensures seedling emergence by regulating ER-mitochondrial interaction and mitophagy.

Nature plants·2026

Search research articles

Related Experiment Video

Updated: May 7, 2025

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

GRAPE-WEB: An automated computational redesign web server for improving protein thermostability.

Jinyuan Sun^1,2, Wenyu Shi³, Zhihui Xing¹

¹AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology Chinese Academy of Sciences Beijing China.

|January 2, 2025

Summary

We developed the GReedy Accumulated strategy for Protein Engineering (GRAPE) to enhance enzyme stability. GRAPE-WEB makes this computational tool user-friendly for designing stabilizing mutations with minimal experiments.

More Related Videos

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

Related Experiment Videos

Last Updated: May 7, 2025

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

Area of Science:

Biochemistry
Computational Biology
Protein Engineering

Background:

Enzyme stability is crucial for various biotechnological applications.
Improving protein thermostability often requires extensive experimental screening.
Understanding epistatic effects is key to rational protein design.

Purpose of the Study:

To introduce a computational strategy, GRAPE, for improving enzyme stability.
To develop an accessible web server, GRAPE-WEB, for protein engineering.
To facilitate the design of stabilizing mutations with reduced experimental effort.

Main Methods:

Developed the GReedy Accumulated strategy for Protein Engineering (GRAPE).
Integrated GRAPE into an automated, user-friendly web server (GRAPE-WEB).
Employed clustering and greedy accumulation to explore epistatic effects.

Main Results:

GRAPE efficiently explores epistatic effects for enzyme stabilization.
GRAPE-WEB enables designing, inspecting, and combining stabilizing mutations.
The web server is accessible to users with varying bioinformatics expertise.

Conclusions:

GRAPE provides a robust approach to protein thermostability design.
GRAPE-WEB enhances accessibility for protein engineering practitioners.
The tool supports both novice and experienced researchers in enzyme stabilization.