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Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

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Backscattering Mueller matrix polarimetry estimates microscale anisotropy and orientation in complex brain tissue

Rhea Carlson¹, Courtney Comrie¹, Justina Bonaventura²

¹University of Arizona, College of Biomedical Engineering, Tucson, Arizona, United States.

Journal of Medical Imaging (Bellingham, Wash.)

|January 2, 2025

Summary

Complete backscattering Mueller matrix polarized light imaging (PLI) shows promise for validating diffusion magnetic resonance imaging (dMRI) microstructural data in thick brain tissues. This advanced PLI technique offers enhanced directional and anisotropy insights.

Keywords:

brain imaging diffusion magnetic resonance imaging multi-modal imaging polarized light imaging validation

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Published on: September 8, 2023

Area of Science:

Neuroimaging
Optical Physics
Biophysics

Background:

Diffusion magnetic resonance imaging (dMRI) is crucial for quantifying brain microstructure.
Diffusion tractography is a key clinical application of dMRI.
Advanced imaging techniques are needed to validate dMRI findings at a microstructural level.

Purpose of the Study:

To compare complete backscattering Mueller matrix polarized light imaging (PLI) with dMRI in ferret optic chiasm (OC) tissue.
To investigate PLI as a dMRI validation tool.
To understand the microstructural and orientational sensitivity of PLI in varying tissue thicknesses.

Main Methods:

Post-mortem ferret brain tissue (whole brain and OC) was imaged using dMRI and complete backscattering Mueller matrix PLI.
Specimens were sectioned and re-imaged with PLI.
Correlation analyses were performed on scalar metrics and orientation vectors from both imaging modalities.

Main Results:

Optical retardance and dMRI fractional anisotropy demonstrated correlated trends, suggesting a macroscale architectural bias in retardance.
Thick tissue analysis revealed comparable orientation between the diattenuation angle (PLI) and dMRI fiber orientation distribution glyphs.
Orientation information from the retardance angle was less consistent.

Conclusions:

Backscattering Mueller matrix PLI is a potential tool for validating dMRI microstructural data in thick tissue specimens.
Complete polarimetry provides directional characterization and microscale anisotropy information beyond conventional PLI.
This study advances the validation of neuroimaging techniques through multimodal comparison.