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Related Experiment Video

Updated: May 7, 2025

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Advanced Lung-on-a-Chip Technology: Mimicking the Complex Human Lung Microenvironment.

Eun-Kyung Min1,2, Choon-Mi Lee1,2, Soo-Rim Kim1,2

  • 1Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon 21999, Republic of Korea.

International Journal of Biological Sciences
|January 2, 2025
PubMed
Summary

This study introduces a novel 3D lung-on-a-chip model that accurately mimics lung tissue complexity. It identifies SERPINB2 as a sensitive biomarker for detecting lung toxicity in drug candidates.

Keywords:
Fluorescence ScreeningLung ToxicityLung-on-a-ChipNatural PolymersSERPINB2

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Area of Science:

  • Biomedical Engineering
  • Pulmonary Medicine
  • Toxicology

Background:

  • Existing in vitro lung models fail to replicate the complex multicellular interactions and microenvironment of native lung tissue.
  • Accurate replication of lung tissue architecture and cellular crosstalk is essential for reliable toxicity testing.

Purpose of the Study:

  • To develop an advanced multicellular 3D lung-on-a-chip system that precisely mimics the pulmonary microenvironment.
  • To identify sensitive biomarkers for early detection of lung toxicity.
  • To create a high-throughput screening platform for evaluating the lung toxicity of drug candidates.

Main Methods:

  • Fabrication of a 3D lung-on-a-chip system using natural polymers (collagen, hyaluronic acid) and blood coagulation factors.
  • Incorporation of diverse lung cells into the microstructured chip.
  • Development of a fluorescence-linked biomarker screening platform to measure toxicity.
  • Exposure of the chip to toxic substances and evaluation of biomarker responses (SERPINB2) and functional indicators.

Main Results:

  • The 3D lung-on-a-chip system accurately replicated the intricate 3D architecture and dynamic microenvironment of the lung.
  • Biomarker responses, specifically SERPINB2 activation in small airway epithelial cells, were more rapid and sensitive indicators of toxicity than cell proliferation or apoptosis.
  • The fluorescence-linked screening platform enabled intuitive and quantitative evaluation of lung toxicity.

Conclusions:

  • The developed lung-on-a-chip platform offers a more physiologically relevant model for studying lung biology and toxicity.
  • SERPINB2 is a promising early biomarker for assessing lung toxicity.
  • This system facilitates efficient and accurate screening of drug candidates for potential lung toxicity.