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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: May 7, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Single-cell spatial proteomics.

Senal Liyanage¹, Jia Guo²

¹Biodesign Institute and School of Molecular Sciences, Arizona State University, Tempe, Arizona, USA.

Histology and Histopathology

|January 2, 2025

Summary

This summary is machine-generated.

Single-cell spatial proteomics, using mass spectrometry imaging and multiplexed immunofluorescence, reveals cellular heterogeneity and protein functions. These technologies advance understanding of biological systems for diagnostics and personalized medicine.

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Last Updated: May 7, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Published on: July 6, 2022

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Published on: April 19, 2019

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Area of Science:

Proteomics
Cellular Biology
Biotechnology

Background:

Single-cell spatial proteomics offers novel insights into cellular signaling networks.
Understanding protein localization and function is crucial for health and disease research.

Purpose of the Study:

To review advancements in single-cell spatial proteomics technologies.
To discuss the applications, challenges, and future directions of these methods.

Main Methods:

Mass spectrometry (MS) imaging for high-resolution, high-dimensional protein profiling.
Multiplexed immunofluorescence (mIF) for rapid, high-throughput protein analysis.

Main Results:

These techniques enable detailed protein profiling at the single-cell level.
Spatial proteomics reveals cellular heterogeneity, organization, and function in native contexts.
MS imaging provides subcellular localization and abundance data; mIF offers speed and accessibility.

Conclusions:

Spatial proteomics is a powerful tool for understanding complex biological systems.
Overcoming current methodological challenges will enhance its utility.
Integration with systems biology promises advancements in diagnostics and personalized treatments.